- add physical_size_z if known but missing from ome.
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@@ -16,7 +16,7 @@ from traceback import print_exc
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import numpy as np
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import ome_types
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import yaml
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from ome_types import model, ureg
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from ome_types import model, ureg, OME
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from pint import set_application_registry
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from tiffwrite import IJTiffFile
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from tqdm.auto import tqdm
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@@ -77,8 +77,11 @@ class DequeDict(OrderedDict):
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def find(obj, **kwargs):
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for item in obj:
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if all([getattr(item, key) == value for key, value in kwargs.items()]):
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return item
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try:
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if all([getattr(item, key) == value for key, value in kwargs.items()]):
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return item
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except AttributeError:
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pass
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def try_default(fun, default, *args, **kwargs):
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@@ -538,11 +541,11 @@ class Imread(np.lib.mixins.NDArrayOperatorsMixin, ABC):
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if self.laserpowers:
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s.append('laser powers: ' + ' | '.join([' & '.join(len(p) * ('{:.3g}',)).format(*[100 * i for i in p])
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for p in self.laserpowers]) + ' %')
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if self.objective:
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if self.objective and self.objective.model:
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s.append(f'objective: {self.objective.model}')
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if self.magnification:
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s.append(f'magnification: {self.magnification}x')
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if self.tubelens:
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if self.tubelens and self.tubelens.model:
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s.append(f'tubelens: {self.tubelens.model}')
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if self.filter:
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s.append(f'filterset: {self.filter}')
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@@ -815,14 +818,28 @@ class Imread(np.lib.mixins.NDArrayOperatorsMixin, ABC):
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return [self.get_channel(c) for c in czt[0]], *czt[1:]
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@staticmethod
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def get_ome(path):
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def get_ome(path: [str, Path]) -> OME:
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""" Use java BioFormats to make an ome metadata structure. """
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with multiprocessing.get_context('spawn').Pool(1) as pool:
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ome = pool.map(get_ome, (path,))[0]
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return ome
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# fix ome if necessary
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for image in ome.images:
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try:
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if image.pixels.physical_size_z is None and len(set([plane.the_z
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for plane in image.pixels.planes])) > 1:
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z = np.array([(plane.position_z * ureg.Quantity(plane.position_z_unit.value).to(ureg.m).magnitude,
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plane.the_z)
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for plane in image.pixels.planes if plane.the_c == 0 and plane.the_t == 0])
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i = np.argsort(z[:, 1])
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image.pixels.physical_size_z = np.nanmean(np.true_divide(*np.diff(z[i], axis=0).T)) * 1e6
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image.pixels.physical_size_z_unit = 'µm'
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except Exception:
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pass
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return ome
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@cached_property
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def ome(self):
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def ome(self) -> OME:
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return self.get_ome(self.path)
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def is_noise(self, volume=None):
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@@ -946,10 +963,6 @@ class AbstractReader(Imread, metaclass=ABCMeta):
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def __frame__(self, c, z, t): # Override this, return the frame at c, z, t
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return np.random.randint(0, 255, self.shape['yx'])
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@cached_property
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def ome(self):
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return self.get_ome(self.path)
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def open(self): # Optionally override this, open file handles etc.
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""" filehandles cannot be pickled and should be marked such by setting do_not_pickle = 'file_handle_name' """
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return
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