- read metadata into ome structure

- pytest
- use pathlib
- series as part of the path: path/PosN
- summary only shows some available metadata
- allow dict in Imread[dict(c=c, z=z, t=t)]
- bfread in different process so the user can start another jvm
- deal with multiple images (series/positions) in czi files
- use jpype instead of javabridge/bioformats
- poetry for install
This commit is contained in:
Wim Pomp
2023-06-29 14:23:03 +02:00
parent 33ddb845ae
commit 506b449f4d
15 changed files with 1268 additions and 920 deletions

View File

@@ -1,10 +1,9 @@
# ndbioimage
# ndbioimage - Work in progress
Exposes (bio) images as a numpy ndarray like object, but without loading the whole
image into memory, reading from the file only when needed. Some metadata is read
and exposed as attributes to the Imread object (TODO: structure data in OME format).
Additionally, it can automatically calculate an affine transform that corrects for
chromatic abberrations etc. and apply it on the fly to the image.
Exposes (bio) images as a numpy ndarray-like-object, but without loading the whole
image into memory, reading from the file only when needed. Some metadata is read and
and stored in an ome structure. Additionally, it can automatically calculate an affine
transform that corrects for chromatic abberrations etc. and apply it on the fly to the image.
Currently supports imagej tif files, czi files, micromanager tif sequences and anything
bioformats can handle.
@@ -13,13 +12,8 @@ bioformats can handle.
pip install ndbioimage@git+https://github.com/wimpomp/ndbioimage.git
### With bioformats (if java is properly installed)
pip install ndbioimage[bioformats]@git+https://github.com/wimpomp/ndbioimage.git
### With affine transforms (only for python 3.8, 3.9 and 3.10)
pip install ndbioimage[transforms]@git+https://github.com/wimpomp/ndbioimage.git
Optionally:
https://downloads.openmicroscopy.org/bio-formats/latest/artifacts/bioformats_package.jar
## Usage
@@ -27,34 +21,34 @@ bioformats can handle.
import matplotlib.pyplot as plt
from ndbioimage import imread
with imread('image_file.tif', axes='ctxy', dtype=int) as im:
from ndbioimage import Imread
with Imread('image_file.tif', axes='ctxy', dtype=int) as im:
plt.imshow(im[2, 1])
- Showing some image metadata
from ndbioimage import imread
from ndbioimage import Imread
from pprint import pprint
with imread('image_file.tif') as im:
with Imread('image_file.tif') as im:
pprint(im)
- Slicing the image without loading the image into memory
from ndbioimage import imread
with imread('image_file.tif', axes='cztxy') as im:
from ndbioimage import Imread
with Imread('image_file.tif', axes='cztxy') as im:
sliced_im = im[1, :, :, 100:200, 100:200]
sliced_im is an instance of imread which will load any image data from file only when needed
sliced_im is an instance of Imread which will load any image data from file only when needed
- Converting (part) of the image to a numpy ndarray
from ndbioimage import imread
from ndbioimage import Imread
import numpy as np
with imread('image_file.tif', axes='cztxy') as im:
with Imread('image_file.tif', axes='cztxy') as im:
array = np.asarray(im[0, 0])
## Adding more formats
@@ -63,9 +57,8 @@ automatically recognize it and use it to open the appropriate file format. Image
subclass Imread and are required to implement the following methods:
- staticmethod _can_open(path): return True if path can be opened by this reader
- \_\_metadata__(self): reads metadata from file and adds them to self as attributes,
- the shape of the data in the file needs to be set as self.shape = (X, Y, C, Z, T)
- other attributes like pxsize, acquisitiontime and title can be set here as well
- property ome: reads metadata from file and adds them to an OME object imported
from the ome-types library
- \_\_frame__(self, c, z, t): return the frame at channel=c, z-slice=z, time=t from the file
Optional methods:
@@ -78,5 +71,5 @@ Optional fields:
for example: any file handles
# TODO
- structure the metadata in OME format tree
- more image formats
- re-implement transforms